Description
The assay makes use of two highly specific monoclonal antibodies: A monoclonal antibody specifi c for prolactin is immobilized onto the microplate and another monoclonal antibody specifi c for a different region of prolactin is conjugated to horse radish peroxidase (HRP). Prolactin from the sample and standards are allowed to bind simultaneously to the plate and to the HRP conjugate. The washing and decanting steps remove any unbound HRP conjugate. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed by the enzymatic reaction is directly proportional to the con-centration of prolactin in the sample. A set of standards is used to plot a standard curve from which the amount of prolactin in patient samples and controls can be directly read